Search for: All records

Abstract Distributed acoustic sensing (DAS) is being explored in a variety of environments as a promising technology for the recording of seismic signals in dense array configurations. There is a particular interest for deploying DAS arrays on the ocean floor, presenting formidable challenges for conventional seismology. Taking advantage of the availability of a dark fiber on the Monterey Bay Accelerated Research System (MARS) 52 km offshore cable at Monterey Bay, California, in July 2022, we installed a DAS interrogator at the shore end of the cable with the intention of acquiring continuous data for a period of one year. Here, we describe the experiment and present examples of observations over the first six months of the deployment. 

Abstract Understanding gene regulatory networks is essential to elucidate developmental processes and environmental responses. Here, we studied regulation of a maize (Zea mays) transcription factor gene using designer transcription activator-like effectors (dTALes), which are synthetic Type III TALes of the bacterial genus Xanthomonas and serve as inducers of disease susceptibility gene transcription in host cells. The maize pathogen Xanthomonas vasicola pv. vasculorum was used to introduce 2 independent dTALes into maize cells to induced expression of the gene glossy3 (gl3), which encodes a MYB transcription factor involved in biosynthesis of cuticular wax. RNA-seq analysis of leaf samples identified, in addition to gl3, 146 genes altered in expression by the 2 dTALes. Nine of the 10 genes known to be involved in cuticular wax biosynthesis were upregulated by at least 1 of the 2 dTALes. A gene previously unknown to be associated with gl3, Zm00001d017418, which encodes aldehyde dehydrogenase, was also expressed in a dTALe-dependent manner. A chemically induced mutant and a CRISPR-Cas9 mutant of Zm00001d017418 both exhibited glossy leaf phenotypes, indicating that Zm00001d017418 is involved in biosynthesis of cuticular waxes. Bacterial protein delivery of dTALes proved to be a straightforward and practical approach for the analysis and discovery of pathway-specific genes in maize. 

Abstract Magma emplacement in the top unconsolidated sediments of rift basins is poorly understood. We compare two shallow sills from the Guaymas Basin (Gulf of California) using core data and analyses from IODP Expedition 385, and high‐resolution 2D seismic data. We show that magma stalling in the top uncemented sediment layer is controlled by the transition from siliceous claystone to uncemented silica‐rich sediment, favoring flat sill formation. Space is created through a combination of viscous indentation, magma‐sediment mingling and fluidization processes. We show that sills emplace above the opal‐A/CT diagenetic barrier. Our model suggests that in low magma input regions sills emplace at constant depth from the seafloor, while high magma input leads to upward stacking of sills, culminating in a funnel‐shaped intrusions. Our petrophysical, petrographic, and textural analyses show that magma‐sediment mingling creates significant porosity (up to 20%) through thermal cracking of the assimilated sediment. Stable isotope data suggest carbonate formation at 70–90°C, consistent with background geothermal gradient at 250–325 m depth. The unconsolidated, water‐rich host sediments produce little thermogenic gas through contact metamorphism, but deep diagenetically formed gas bypasses the low‐permeability top sediments via hydrothermal fluids flowing through the magma plumbing system. This hydrothermal system provides a steady supply of hydrocarbons at temperatures amendable for microbial life, serving as an incubator that may be abundant in magma‐rich young rift basins and play a key role in sustaining subseafloor ecosystems. 

Plants are vulnerable to disease through pathogen manipulation of phytohormone levels, which otherwise regulate development, abiotic, and biotic responses. Here, we show that the wheat pathogen Xanthomonas translucens pv. undulosa elevates expression of the host gene encoding 9- cis -epoxycarotenoid dioxygenase ( TaNCED-5BS ), which catalyzes the rate-limiting step in the biosynthesis of the phytohormone abscisic acid and a component of a major abiotic stress-response pathway, to promote disease susceptibility. Gene induction is mediated by a type III transcription activator-like effector. The induction of TaNCED-5BS results in elevated abscisic acid levels, reduced host transpiration and water loss, enhanced spread of bacteria in infected leaves, and decreased expression of the central defense gene TaNPR1 . The results represent an appropriation of host physiology by a bacterial virulence effector.